• Print
  • Download PDF

Adenovirus Quantitative Real-time PCR

Test Code: 7500

Cpt Code:

87799 (x1)

Clinical Utility

Adenovirus is an important cause of morbidity and mortality in the transplant setting, causing pneumonia, hemorrhagic cystitis, hepatitis, encephalitis, pancreatitis, enteritis, and disseminated disease with the mortality rate reaching 60% in some especially high risk situations such as pediatric hematopoietic stem cell transplantation. Since proper management is dependent upon early diagnosis, quantitative adenovirus DNA PCR is useful for detecting the virus, tracking the course of infection, and monitoring response to treatment.

Procedure

Extraction of adenovirus DNA from specimen followed by amplification and detection of known adenovirus strains using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. Viracor-IBT's assay design includes multiple targets to account for viral mutations, which significantly reduces the chance of false negative results. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Specificity

Detects all known serotypes in one assay. The primers and probes used in this assay are specific for known adenovirus strains based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, Parvovirus B19, SV-40, and VZV.

Causes For Rejection

Wood shafted swab, calcium alginate swab, specimens received in trap containers, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Turnaround Time

Same day, (within 8 to 12 hours of receiving specimen) Monday through Saturday.

Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor-IBT test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor-IBT Laboratories, 1001 NW Technology Dr, Lee's Summit, MO 64086

Specimen Information

7509 BAL

NY approved.  Assay Range: 38 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7504 bone marrow

NY approved.  Assay Range: 589 copies/mL to 1x1010 copies/mL. 2 mL collected in EDTA tube (minimum volume 0.2 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Do not centrifuge. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7526 bronch wash

NY approved.  Assay Range: 38 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7503 CSF

NY approved.  Assay Range: 109 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

7508 fecal

NY approved.  (CPT Code:87798 X 1) Assay Range: (Detected/Not Detected). Collect small amount of fecal material (size of pea, or 2 mL liquid stool) and place into screw top tube for shipment. Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

7531 nasal asp

NY approved.  Assay Range: 54 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7530 nasal swab

NY approved.  (CPT Code:87798 X 1) Assay Range: (Detected/Not Detected). Sterile swab placed in 2 mL sterile saline, M4, or viral transport media in a sterile, screw top tube. Do not use calcium alginate swab or wood shafted swab. Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7513 nasal wash

NY approved.  Assay Range: 54 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7524 NP aspirate

NY approved.  Assay Range: 54 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7525 NP swab

NY approved.  (CPT Code:87798 X 1) Assay Range: (Detected/Not Detected). Sterile swab placed in 2 mL sterile saline, M4, or viral transport media in a sterile, screw top tube. Do not use calcium alginate swab or wood shafted swab. Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7547 NP wash

NY approved.  Assay Range: 54 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7512 pericardial fluid

NY approved.  Assay Range: 157 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Ship Monday through Friday. Specimens shipped at ambient temperature must be received less than 48 hours from collection. Specimens greater than 48 hours from draw will be accepted if they have been stored frozen and shipped on dry ice overnight to Viracor-IBT.

7501 plasma

NY approved.  Assay Range: 190 copies/mL to 1x1010 copies/mL. Collect 4-5 mL whole blood in EDTA or ACD tube, centrifuge and transfer 2 mL plasma to sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7511 pleural fluid

NY approved.  Assay Range: 157 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Ship Monday through Friday. Specimens shipped at ambient temperature must be received less than 48 hours from collection. Specimens greater than 48 hours from collection will be accepted if they have been stored frozen and shipped on dry ice overnight to Viracor-IBT.

7510 serum

NY approved.  Assay Range: 190 copies/mL to 1x1010 copies/mL. Collect 4-5 mL whole blood in red top tube, centrifuge and transfer 2 mL serum to sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7523 throat gargle

NY approved.  Assay Range: 54 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7506 tissue [Qual]

NY approved.  (CPT Code:87798 X 1) Assay Range: (Detected/Not Detected). Place fresh tissue in a sterile, screw top container. The preferred handling is to not add water, saline or other fluid media to the tissue container; however, fluid media filled containers will be accepted for qualitative results. Store frozen and ship on dry ice for overnight delivery to Viracor-IBT. Formalin fixed, paraffin embedded tissue will not be accepted.

7505 tissue [Quant]

NY approved.  Assay Range: 13 copies/mg to 1x109 copies/mg. Place a minimum of 5 mg fresh tissue (approximately ½ of a pencil eraser size) in a sterile, screw top container. Do not add water, saline or other fluid media to container. Store frozen and ship on dry ice for overnight delivery to Viracor-IBT. Formalin fixed, paraffin embedded tissue will not be accepted.

7519 trach asp

NY approved.  Assay Range: 38 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7548 trach wash

NY approved.  Assay Range: 38 copies/mL to 1x1010 copies/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

7502 urine

NY approved.  Assay Range: 152 copies/mL to 1x1010 copies/mL. 2 mL sample collected in a sterile urinalysis container then transferred to sterile, screw top tube for shipment (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

Disclaimer

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor-IBT's interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor-IBT assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.

<< Back to list