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Eosinophil-Derived Neurotoxin (EDN) Serum

Test Code: 30058

Cpt Code:

83520 (x1)

Clinical Utility

Upon activation, eosinophils secrete various biomarkers including EDN. Due to its important role in the inflammatory process of allergic diseases, EDN is an important marker of Eosinophil activation and degranulation. Additonally, elevated EDN levels can signify food allergy, parasitosis, and general intestinal pathogen invasion.

Procedure

Sandwich ELISA technique. Serum EDN binds to the EDN specific monoclonal antibody that is pre-coated to a micro plate. Bound EDN is detected by an enzyme-linked EDN specific monoclonal antibody. Substrate solution is added and color develops in proportion to the amount of EDN in the sample. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Assay Range

The result is reported in ng/mL. The assay range is approximately 3 to 200. The reference range for a healthy population is 8.5 – 46.7 ng/mL. However it should be noted that these ranges are obtained from a limited population of apparently healthy adults and are not diagnostic thresholds.

Turnaround Time

Set up M, W, F. TAT 3-5 days from receipt of specimen

Specimen Information

serum - NY approved. 1 mL. Blood should be allowed to clot prior to centrifugation. Specimens should be frozen (-70°C). Ship overnight on dry ice.

Stability
Frozen, stable 3 freeze/thaw cycles

Disclaimer

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor-IBT's interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for general informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor-IBT assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

References

Durack D.T., et al. Proc. Natl. Acad. Sci. USA 78, 5165 (1981).

Peterson C.G., et al. Immunology 50, 19 (1983).

Slifman C.G., et al. J. Immunol. 143, 2317 (1989).

Gullberg U., et al. Biochem. Biophis. Res. Commun. 139, 1239 (1986).

Slifman N.R., et al. J. Immunol. 137, 2913 (1986).

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