Test Information

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Neutrophil Oxidative Burst Test

Test Code: 403002P

Cpt Code:

82657 (x1)

Clinical Utility

The measure of oxidation is a useful assay in the diagnosis of chronic granulomatous disease and is also a useful means to determine the overal metabolic integrity of phagocytizing neutrophils.


Formation of the reactive oxidants during the oxidative burst is monitored by the addition and enzymatic oxidation of a fluorogenic substrate, DHR 123. The level of reactive oxygen radicals is determined by flow cytometry. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Causes For Rejection

Specimens greater than 36 hours old, whole blood frozen, received in transfer tube, or hemolyzed samples. Specimen type other than heparinized whole blood in sodium herparin tube.

Turnaround Time

2-3 business days from receipt of specimen

Specimen Information

whole blood

5 mL, whole blood collected and shipped in original sodium heparin (green top) tube. Ship at ambient temperature, priority overnight Monday through Friday. Specimen must be shipped on same day as collection to meet 36 hour requirement. In addition, it is recommended that a control specimen be included from a healthy individual as a shipping control.


Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor-IBT's interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for general informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor-IBT assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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