• Print
  • Download PDF

Parvovirus B19 Quantitative Real-time PCR

Test Code: 1500

Cpt Code:

87799 (x1)

Clinical Utility

Parvovirus B19 manifests itself as an acute or chronic hematological disorder in immunocompromised patients. It can cause persistent anemia, sometimes associated with leukopenia and thrombocytopenia. Pediatric transplant patients are at risk for chronic infections, which can be associated with lung and/or renal disorders. Quantitative DNA PCR can be used to detect the presence of the virus, track the course of infection, and monitor response to treatment.

Procedure

Extraction of parvovirus B19 viral DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Specificity

The primers and probes used in this assay are specific for parvovirus B19 based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, SV-40, and VZV.

Causes For Rejection

Specimens beyond their acceptable length of time from collection as listed in the specimen handling, specimens received in trap containers or specimen types other than those listed.

Turnaround Time

Same day (within 8 to 12 hours of receiving specimen), Monday through Saturday

Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor-IBT test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor-IBT Laboratories, 1001 NW Technology Dr, Lee's Summit, MO 64086

Specimen Information

1507 amniotic fluid

NY approved.  Assay Range: 105 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

1509 BAL

NY approved.  Assay Range: 59 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

1504 bone marrow

NY approved.  Assay Range: 145 IU/mL to 1.38x1010 IU/mL. 2 mL collected in EDTA tube (minimum volume 0.2 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

1526 bronch wash

NY approved.  Assay Range: 59 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

1503 CSF

NY approved.  Assay Range: 246 IU/mL to 1.38x1010 IU/mL. 2 mL, collected in a sterile, screw top tube (minimum volume 0.5 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

1512 pericardial fluid

NY approved.  Assay Range: 105 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

1501 plasma

NY approved.  Assay Range: 199 IU/mL to 1.38x1010 IU/mL. Collect 4-5 mL whole blood in EDTA or ACD tube, centrifuge and transfer 2 mL plasma to sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

1511 pleural fluid

NY approved.  Assay Range: 105 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

1510 serum

NY approved.  Assay Range: 199 IU/mL to 1.38x1010 IU/mL. Collect 4-5 mL whole blood in red top tube, centrifuge and transfer 2 mL serum to sterile, screw top tube (minimum volume 0.5 mL). Can be shipped at ambient or frozen temperature Monday through Friday. Specimens shipped at ambient temperature must be received within 96 hrs of collection.

1506 tissue [Qual]

NY approved.  (CPT Code:87798 X 1). Assay Range: (Detected/Not Detected). Place fresh tissue in a sterile, screw top container. The preferred handling is to not add water, saline or other fluid media to the tissue container; however, fluid media filled containers will be accepted for qualitative results. Store frozen and ship on dry ice for overnight delivery to Viracor-IBT. Formalin fixed, paraffin embedded tissue will not be accepted.

1505 tissue [Quant]

NY approved.  Assay Range: 83 IU/mg to 1.38x109 IU/mg. Place a minimum of 5 mg fresh tissue (approximately ½ of a pencil eraser size) in a sterile, screw top container. Do not add water, saline or other fluid media to container. Store frozen and ship on dry ice for overnight delivery to Viracor-IBT. Formalin fixed, paraffin embedded tissue will not be accepted.

1519 trach asp

NY approved.  Assay Range: 59 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

1548 trach wash

NY approved.  Assay Range: 59 IU/mL to 1.38x1010 IU/mL. 2 mL collected in a sterile, screw top tube (minimum volume 0.5 mL). Store frozen and ship on dry ice for overnight delivery to Viracor-IBT.

Disclaimer

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor-IBT's interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor-IBT assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.

<< Back to list