Test Information

  • Print
  • Download PDF

T Cell Fx™-Complete

Test Code: 403066P

Cpt Code:

86353 (x7)

Clinical Utility

The test measures the T cell responses to antigens of common infections as well as to mitogens, allowing detection of deficiencies in this arm of the immune response. Patients with primary immunodeficiency may have deficits in the acquired T-cell response. It is useful for the following types of patients: Immune deficiency, Reccurent infection, Evaluation of cell-mediated immunity and monitoring immunosuppression (e.g. Transplant patients).

Procedure

Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37ºC. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, and CD69 and intracellular IFN-gamma, and analyzed by flow cytometry. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Turnaround Time

2-3 business days from receipt of specimen

Specimen Information

whole blood

8 mL of whole blood. Collect and ship in original sodium heparin vacutainer. (DO NOT USE lithium heparin, ACD tubes or EDTA anticoagulants.) Due to time constraints of the T Cell FX™ assay, samples need to be collected between the hours of 12 p.m. and 5 p.m. Central time, Monday thru Thursday. Ship samples via priority overnight courier at ambient temperature. DO NOT SHIP on days where a holiday follows the shipping or set up day.

Disclaimer

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor-IBT's interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for general informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor-IBT assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

<< Back to list