Skip to the content

Adenovirus Quantitative Real-time PCR

Test Code: 7500
Expand All Collapse All
Clinical Utility

Adenovirus is an important cause of morbidity and mortality in the transplant setting, causing pneumonia, hemorrhagic cystitis, hepatitis, encephalitis, pancreatitis, enteritis, and disseminated disease with the mortality rate reaching 60% in some especially high risk situations such as pediatric hematopoietic stem cell transplantation. Since proper management is dependent upon early diagnosis, quantitative adenovirus DNA PCR is useful for detecting the virus, tracking the course of infection, and monitoring response to treatment.

Procedure

Extraction of adenovirus DNA from specimen followed by amplification and detection of known adenovirus strains using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. Viracor Eurofins' assay design includes multiple targets to account for viral mutations, which significantly reduces the chance of false negative results. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Specificity

Detects all known serotypes in one assay. The primers and probes used in this assay are specific for known adenovirus strains based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, Parvovirus B19, SV-40, and VZV.

Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
BAL 7509 87799 Yes

2 mL (min. 0.5 mL)

38 copies/mL to 1x1010 copies/mL

  • Collect in a sterile, screw top tube.
  • Ship at ambient or frozen temperature.
  • Specimens shipped ambient temperature must be received within 96 hours of collection.
bone marrow 7504 87799 Yes 2 mL (min. 0.2 mL) 589 copies/mL to 1x1010 copies/mL
  • Collect in EDTA tube.
  • Do not centrifuge.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
bronch wash 7526 87799 Yes 2 mL (min. 0.5 mL) 38 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
CSF 7503 87799 Yes 2 mL (min. 0.5 mL) 109 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.
fecal 7508 87798 Yes Size of pea, or 2 mL liquid stool Detected/Not Detected
  • Collect and place in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.
nasal asp 7531 87799 Yes 2 mL (min. 0.5 mL) 54 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
nasal swab 7530 87798 Yes 2 mL Detected/Not Detected
  • Place sterile swab placed in 2 mL sterile saline, M4, or viral transport media in a sterile, screw top tube.
  • Do not use calcium alginate swab or wood shafted swab.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
nasal wash 7513 87799 Yes 2 mL (min. 0.5 mL) 54 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
NP aspirate 7524 87799 Yes 2 mL (min. 0.5 mL) 54 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
NP swab 7525 87798 Yes 2 mL Detected/Not Detected
  • Place sterile swab placed in 2 mL sterile saline, M4, or viral transport media in a sterile, screw top tube.
  • Do not use calcium alginate swab or wood shafted swab.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
NP wash 7547 87799 Yes 2 mL (min. 0.5 mL) 54 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
pericardial fluid 7512 87799 Yes 2 mL (min. 0.5 mL) 157 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Ship Monday through Friday.
  • Specimens shipped at ambient temperature must be received less than 48 hours from collection.
  • Specimens greater than 48 hours from draw will be accepted if they have been stored frozen and shipped on dry ice overnight.
plasma 7501 87799 Yes 2 mL (min. 0.5 mL) 190 copies/mL to 1x1010 copies/mL
  • Collect 4-5 mL whole blood in EDTA or ACD tube.
  • Centrifuge and transfer 2 mL plasma to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
pleural fluid 7511 87799 Yes 2 mL (min. 0.5 mL) 157 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Ship Monday through Friday.
  • Specimens shipped at ambient temperature must be received less than 48 hours from collection.
  • Specimens greater than 48 hours from draw will be accepted if they have been stored frozen and shipped on dry ice overnight.
serum 7510 87799 Yes 2 mL (min. 0.5 mL) 190 copies/mL to 1x1010 copies/mL
  • Collect 4-5 mL whole blood in red top tube.
  • Centrifuge and transfer 2 mL serum to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
throat gargle 7523 87799 Yes

2 mL (min. 0.5 mL)

54 copies/mL to 1x1010 copies/mL

  • Collect 2 mL in sterile, screw top tube
  • Centrifuge and transfer 2 mL serum to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
tissue [Qual] 7506 87798 Yes 5 mg fresh tissue (approximately ½ of a pencil eraser size) Detected/Not Detected
  • Place fresh tissue in a sterile, screw top container.
  • The preferred handling is to not add water, saline or other fluid media to the tissue container; however, fluid media filled containers will be accepted for qualitative results.
  • Store frozen and ship on dry ice for overnight delivery.
  • Formalin fixed, paraffin embedded tissue will not be accepted.
tissue [Quant] 7505 87799 Yes 5 mg fresh tissue (approximately ½ of a pencil eraser size) 13 copies/mL to 1x1010 copies/mL
  • Place fresh tissue in a sterile, screw top container.
  • Do not add water, saline or other fluid media to container.
  • Store frozen and ship on dry ice for overnight delivery.
  • Formalin fixed, paraffin embedded tissue will not be accepted.
trach asp 7519 87799 Yes 2 mL (min. 0.5 mL) 38 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
trach wash 7548 87799 Yes 2 mL (min. 0.5 mL) 38 copies/mL to 1x1010 copies/mL
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
urine 7502 87799 Yes 2 mL (min. 0.5 mL) 152 copies/mL to 1x1010 copies/mL
  • Collect in a sterile urinalysis container then transfer to sterile, screw top tube for shipment
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086

Causes for Rejection

Wood shafted swab, calcium alginate swab, specimens received in trap containers, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.

Connect with us