Skip to the content

BK Virus (BKV) Quantitative Real-time PCR

Test Code: 2500
Expand All Collapse All
Clinical Utility

BKV has emerged as an important pathogen in nephropathy in kidney transplant patients and hemorrhagic cystitis in hematopoietic stem cell transplantation patients. Early diagnosis of BK nephropathy has been shown to positively impact organ survival. Early diagnosis can be accomplished through a regular monitoring program for reactivation of BKV. Monitoring is effectively accomplished through the use of quantitative BKV DNA PCR of both blood and urine specimens. Quantitative PCR can also be used to track the course of infection and monitor response to treatment.

Procedure

Extraction of BK Viral DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. Viracor Eurofins' assay design includes multiple targets to account for viral mutations, which significantly reduces the chance of false negative results. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Specificity

Designed to detect all strains of BKV. The primers and probes used in this assay are specific for all known BKV strains based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, CMV, EBV, HSV-1, HSV-2, HHV-6, HHV-7, HHV-8, JCV, parvovirus B19, SV-40, and VZV.

Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
CSF 2503 87799 Yes 2 mL (min. 0.5 mL)

52 copies/mL to 1x1010 copies/mL

  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.
plasma 2501 87799 Yes 2 mL (min. 0.5 mL) 39 copies/mL to 1x1010 copies/mL
  • Collect 4-5 mL whole blood in EDTA or ACD tube.
  • Centrifuge and transfer 2 mL plasma to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
serum 2510 87799 Yes

2 mL (min. 0.5 mL)

39 copies/mL to 1x1010 copies/mL

  • Collect 4-5 mL whole blood in red top tube or SST.
  • Centrifuge and transfer 2 mL serum to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
tissue [Qual] 2506 87798 Yes 5 mg fresh tissue (approximately ½ of a pencil eraser size) Detected/Not Detected
  • Place fresh tissue in a sterile, screw top container.
  • The preferred handling is to not add water, saline or other fluid media to the tissue container; however, fluid media filled containers will be accepted for qualitative results.
  • Store frozen and ship on dry ice for overnight delivery.
  • Formalin fixed, paraffin embedded tissue will not be accepted.
tissue [Quant] 2505 87799 Yes 5 mg fresh tissue (approximately ½ of a pencil eraser size)

56 copies/mg to 1x109 copies/mg

  • Place fresh tissue in a sterile, screw top container.
  • Do not add water, saline or other fluid media to container.
  • Store frozen and ship on dry ice for overnight delivery.
  • Formalin fixed, paraffin embedded tissue will not be accepted.
urine 2502 87799 Yes 2 mL (min. 0.5 mL) 500 copies/mL to 1x1010 copies/mL
  • Collect in a sterile urinalysis container then transfer to sterile, screw top tube for shipment
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling or specimens other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.

Connect with us