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Parvovirus B19 Quantitative Real-time PCR
Test Code: 1500
Some specimen types for this assay are reported as qualitative results; please see our Specimen Information section below for more information.
Clinical Utility
Parvovirus B19 manifests itself as an acute or chronic hematological disorder in immunocompromised patients. It can cause persistent anemia, sometimes associated with leukopenia and thrombocytopenia. Pediatric transplant patients are at risk for chronic infections, which can be associated with lung and/or renal disorders. Quantitative DNA PCR can be used to detect the presence of the virus, track the course of infection, and monitor response to treatment.
Procedure
Extraction of parvovirus B19 viral DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
Specificity
The primers and probes used in this assay are specific for parvovirus B19 based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, SV-40, and VZV.
Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.
| Specimen Type | Order Code | CPT Code | NY Approved | Volume | Assay Range | Special Instructions |
|---|---|---|---|---|---|---|
| amniotic fluid | 1507 | 87799 | Yes | 2 mL (min. 0.5 mL) |
105 IU/mL to 1.38x1010 IU/mL. |
|
| BAL | 1509 | 87799 | Yes | 2 mL (min. 0.5 mL) |
59 IU/mL to 1.38x1010 IU/mL |
|
| bone marrow | 1504 | 87799 | Yes | 2 mL (min. 0.5 mL) |
145 IU/mL to 1.38x1010 IU/mL |
|
| bronch wash | 1526 | 87799 | Yes | 2 mL (min. 0.5 mL) |
59 IU/mL to 1.38x1010 IU/mL |
|
| CSF | 1503 | 87799 | Yes | 2 mL (min. 0.5 mL) |
246 IU/mL to 1.38x1010 IU/mL |
|
| pericardial fluid | 1512 | 87799 | Yes | 2 mL (min. 0.5 mL) |
105 IU/mL to 1.38x1010 IU/mL. |
|
| plasma | 1501 | 87799 | Yes | 2 mL (min. 0.5 mL) |
199 IU/mL to 1.38x1010 IU/mL. |
|
| pleural fluid | 1511 | 87799 | Yes | 2 mL (min. 0.5 mL) |
105 IU/mL to 1.38x1010 IU/mL. |
|
| serum | 1510 | 87799 | Yes | 2 mL (min. 0.5 mL) |
199 IU/mL to 1.38x1010 IU/mL. |
|
| tissue [Qual] | 1506 | 87798 | Yes | 5 mg fresh tissue (approximately ½ of a pencil eraser size) | Detected/Not Detected |
|
| tissue [Quant] | 1505 | 87799 | Yes | 5 mg fresh tissue (approximately ½ of a pencil eraser size) | 83 IU/mg to 1.38x109 IU/mg |
|
| trach asp | 1519 | 87799 | Yes | 2 mL (min. 0.5 mL) |
59 IU/mL to 1.38x1010 IU/mL |
|
| trach wash | 1548 | 87799 | Yes | 2 mL (min. 0.5 mL) |
59 IU/mL to 1.38x1010 IU/mL |
|
Shipping
Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086
Causes for Rejection
Specimens beyond their acceptable length of time from collection as listed in the specimen handling, specimens received in trap containers or specimen types other than those listed.
Specimens are approved for testing in New York only when indicated in the Specimen Information field above.
The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.