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Parvovirus B19 Quantitative Real-time PCR

Test Code: 1500

Some specimen types for this assay are reported as qualitative results; please see our Specimen Information section below for more information.

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Clinical Utility

Parvovirus B19 manifests itself as an acute or chronic hematological disorder in immunocompromised patients. It can cause persistent anemia, sometimes associated with leukopenia and thrombocytopenia. Pediatric transplant patients are at risk for chronic infections, which can be associated with lung and/or renal disorders. Quantitative DNA PCR can be used to detect the presence of the virus, track the course of infection, and monitor response to treatment.

Procedure

Extraction of parvovirus B19 viral DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Specificity

The primers and probes used in this assay are specific for parvovirus B19 based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, SV-40, and VZV.

Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
amniotic fluid 1507 87799 Yes

2 mL (min. 0.5 mL)

105 IU/mL to 1.38x1010 IU/mL.
  • Collect 2 mL in sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped ambient temperature must be received within 96 hours of collection
BAL 1509 87799 Yes

2 mL (min. 0.5 mL)

59 IU/mL to 1.38x1010 IU/mL
  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.

 
bone marrow 1504 87799 Yes

2 mL (min. 0.5 mL)

145 IU/mL to 1.38x1010 IU/mL
  • Collect in EDTA tube.
  • Store frozen and ship on dry ice for overnight delivery.

 
bronch wash 1526 87799 Yes

2 mL (min. 0.5 mL)

59 IU/mL to 1.38x1010 IU/mL
  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.
CSF 1503 87799 Yes

2 mL (min. 0.5 mL)

246 IU/mL to 1.38x1010 IU/mL
  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.
pericardial fluid 1512 87799 Yes

2 mL (min. 0.5 mL)

105 IU/mL to 1.38x1010 IU/mL.
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
plasma 1501 87799 Yes

2 mL (min. 0.5 mL)

199 IU/mL to 1.38x1010 IU/mL.
  • Collect 4-5 mL whole blood in EDTA or ACD tube.
  • Avoid using gel separator tubes; samples collected in gel separator tubes have increased rate of PCR inhibition.
  • Centrifuge and transfer 2 mL plasma to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
pleural fluid 1511 87799 Yes

2 mL (min. 0.5 mL)

105 IU/mL to 1.38x1010 IU/mL.
  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
serum 1510 87799 Yes

2 mL (min. 0.5 mL)

199 IU/mL to 1.38x1010 IU/mL.
  • Collect 4-5 mL whole blood in red top tube.
  • Avoid using gel separator tubes; samples collected in gel separator tubes have increased rate of PCR inhibition.
  • Centrifuge and transfer 2 mL serum to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 96 hrs. of collection.
tissue [Qual] 1506 87798 Yes 5 mg fresh tissue (approximately ½ of a pencil eraser size) Detected/Not Detected
  • Place fresh tissue in a sterile, screw top container.
  • The preferred handling is to not add water, saline or other fluid media to the tissue container; however, fluid media filled containers will be accepted for qualitative results.
  • Store frozen and ship on dry ice for overnight delivery.
  • Formalin fixed, paraffin embedded tissue will not be accepted.
tissue [Quant] 1505 87799 Yes 5 mg fresh tissue (approximately ½ of a pencil eraser size) 83 IU/mg to 1.38x109 IU/mg
  • Place fresh tissue in a sterile, screw top container.
  • Do not add water, saline or other fluid media to container.
  • Store frozen and ship on dry ice for overnight delivery.
  • Formalin fixed, paraffin embedded tissue will not be accepted.
trach asp 1519 87799 Yes

2 mL (min. 0.5 mL)

59 IU/mL to 1.38x1010 IU/mL
  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.
trach wash 1548 87799 Yes

2 mL (min. 0.5 mL)

59 IU/mL to 1.38x1010 IU/mL

 

  • Collect in a sterile, screw top tube.
  • Store frozen and ship on dry ice for overnight delivery.

 
Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086

Causes for Rejection

Specimens beyond their acceptable length of time from collection as listed in the specimen handling, specimens received in trap containers or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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