Toxoplasma gondii Quantitative Real-time PCR
Toxoplasma gondii is highly problematic when primary infection occurs during the first trimester of pregnancy, often leading to fetal death or when reactivation occurs in immunocompromised patients, leading to life-threatening disease including encephalitis and extracerebral toxoplasmosis. Onset of disease in most patients is preceded by an increase in parasite load. Monitoring blood levels of Toxoplasma gondii DNA correlates well with treatment.
Extraction of Toxoplasma gondii DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
The primers and probes used in this assay are specific for known Toxoplasma gondii strains based on similarity search algorithms. Additionally, no cross reactivity was detected with any viral or protozoa pathogens.
Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.
|Specimen Type||Order Code||CPT Code||NY Approved||Volume||Assay Range||Special Instructions|
|amniotic fluid||2207||87799||Yes||2 mL (min. 0.5 mL)||266 copies/mL to 1x108 copies/mL||
|aqueous fluid||2245||87799||Yes||1 mL (min. 0.05 mL)||1,423 copies/mL to 1x108 copies/mL||
|BAL||2209||87799||Yes||2 mL (min. 0.5 mL)||212 copies/mL to 1x108 copies/mL||
|bronch wash||2226||87799||Yes||2 mL (min. 0.5 mL)||212 copies/mL to 1x108 copies/mL||
|CSF||2203||87799||Yes||2 mL (min. 0.5 mL)||183 copies/mL to 1x108 copies/mL||
|pericardial fluid||2212||87799||Yes||2 mL (min. 0.5 mL)||266 copies/mL to 1x108 copies/mL||
|pleural fluid||2211||87799||Yes||2 mL (min. 0.5 mL)||266 copies/mL to 1x108 copies/mL||
|trach asp||2219||87799||Yes||2 mL (min. 0.5 mL)||212 copies/mL to 1x108 copies/mL||
|trach wash||2248||87799||Yes||2 mL (min. 0.5 mL)||212 copies/mL to 1x108 copies/mL||
|vitreous fluid||2214||87799||Yes||1 mL (min. 0.05 mL)||1,423 copies/mL to 1x108 copies/mL||
|whole blood||2216||87799||Yes||2 mL (min. 0.5 mL)||376 copies/mL to 1x108 copies/mL||
Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086
Specimens beyond their acceptable length of time from collection as listed in the specimen handling, specimens received in trap containers or specimen types other than those listed.
Specimens are approved for testing in New York only when indicated in the Specimen Information field above.
The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.
PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.