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Strongyloides Antibody IgG EIA

Test Code: 30826

A special account is required to order pre-transplant testing. Contact Client Services or your account executive to set up a pre-transplant account to order this assay. Specimens should not be collected until after account has been created.

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Clinical Utility

This Strongyloides ELISA test is a qualitative enzyme immunoassay for the detection of antibodies to Strongyloides, in samples of human serum or plasma. This test is intended to be performed by trained medical technologists only.

About Strongyloides

Strongyloidiasis is the disease caused by the Strongyloides stercoralis parasite. This organism is an intestinal nematode with worldwide distribution, but is especially common in tropical and subtropical regions. The disease usually manifests as intestinal symptoms (mild diarrhea). In a minority of cases, the organism will become extra-intestinal and may lead to septic shock and meningitis.

Serological tests are useful in detecting infection by Strongyloides if the organism goes extra-intestinal and in excluding the organism from the diagnosis of other disorders (especially hematologic malignancies). Strongyloides infected patients are particularly at risk for severe complications if they are also immunocompromised.

Procedure

The microwells are coated with Strongyloides L3 antigen. During the first incubation with the diluted patients’ sera, any antibodies which are reactive with the antigen will bind to the coated wells. After washing to remove the rest of the sample, the Enzyme Conjugate is added. If antibodies have been bound to the wells, the Enzyme Conjugate will then bind to these antibodies. After another series of washes, a chromogen (tetramethylbenzidine or TMB) is added. If the Enzyme Conjugate is present, the peroxidase will catalyze a reaction that consumes the peroxide and turns the chromogen from clear to blue. Addition of the Stop Solution ends the reaction and turns the blue color to a bright yellow color. The reaction may then be read visually or with an ELISA reader. Test performed by VRL Eurofins - Los Angeles, 2100 West 3rd Street, Suite 301, Los Angeles, CA. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration. See package insert for more information.

1-3 business days from receipt of specimen.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
Plasma 30826 86682 Yes

300 µL (min 100µL)

Qualitative

  • Collect whole blood in an EDTA, lavender top tube. ACD, Lithium Heparin, Sodium Citrate, or Sodium Heparin tubes are also accepted. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped ambient temperature when received within 7 days, or refrigerated when received within 14 days.
  • If not shipping in original container, centrifuge and transfer 300 µL (min 100 µL) plasma to screw top tube. Specimens shipped at ambient temperature when received within 7 days, refrigerated received within 14 days, or frozen received within 30 days.
Serum 30826 86682 Yes

300 µL (min 100µL)

Qualitative

  • Collect whole blood in an gold top, red top, or SST tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped ambient temperature when received within 7 days, or refrigerated received within 14 days.
  • If not shipping in original container, centrifuge and transfer 300 µL (min 100 µL) plasma to screw top tube. Specimens shipped at ambient temperature when received within 7 days, refrigerated received within 14 days, or frozen received within 30 days.
Shipping

All specimens must be labeled with patient's name and collection date. A Viracor/VRL Eurofins Pre-Transplant test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112.

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on VRL Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. VRL Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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