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Toxoplasma (Toxo) IgG EIA

Test Code: 30827

Please contact Client Services to set up a Pre-transplant account to order this assay.

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Clinical Utility

For the qualitative, semi-quantitative, or quantitative detection of human IgG antibodies to Toxoplasma gondii in human serum by enzyme immunoassay, as an aid in the determination of infection with Toxoplasma. When used as a qualitative test, Toxoplasma IgG EIA aids in the assessment of the patient's immunological response to Toxoplasma

About Toxoplasmosis

Serologic studies indicate that infection with Toxoplasma gondii, an intracellular parasite and the causative agent of toxoplasmosis, is fairly widespread in the population worldwide. For example, it has been estimated that 30% of the population in the United States exhibits serological evidence of exposure to Toxoplasma gondii.1 The organism can be transmitted during organ transplantation 2, by blood or leukocyte transfusion 3, contact with contaminated cat feces 4, or by ingestion of raw or undercooked meat from infected animals.5

In adults the infection is usually asymptomatic, although symptomatic as well as fatal cases do occur. Symptoms range from swollen lymph nodes to those resembling infectious mononucleosis.1 In children, the disease may affect the central nervous system and the viscera. Congenital infection also occurs, and toxoplasmosis is a significant cause of mortality and congenital malformation. 6-9

Specific IgG antibody titers directed against Toxoplasma gondii prior to pregnancy are correlated with immunity to infection.3 Inasmuch as infection may occur in utero if serologically negative women become infected during pregnancy, it is advisable for pregnant women to be tested for Toxoplasma specific antibodies early during their pregnancy, and serologically negative women should be monitored for Toxoplasma IgG antibody during their pregnancy and at delivery. Serologically positive results should be followed-up by testing for Toxoplasma specific IgM in the newborn. Because less than one percent of newborns are born with maternally transferred IgM, the presence of Toxoplasma specific IgM antibodies is an indication of toxoplasmosis. 10

The results of serologic tests are of value as presumptive evidence of toxoplasmosis. The Toxoplasma IgG EIA test in intended for the detection of IgG antibodies to Toxoplasma. Test results are obtained after one and one-half hours incubation time. They are objective and normalized as Index values, or as International Units (IU/mL), which are traceable to the WHO Anti-Toxoplasma Serum, 3rd International Standard Preparation, 1994.

Procedure

Diluted samples are incubated in antigen-coated wells. Toxoplasma antibodies (if present) are immobilized in the wells. Residual sample is eliminated by washing and draining, and conjugate (enzyme labeled antibodies to human IgG) is added and incubated. If IgG antibodies to Toxoplasma are present, the conjugate will be immobilized in the wells. Residual conjugate is eliminated by washing and draining, and the substrate is added and incubated. In the presence of the enzyme, the substrate is converted to a yellow end product which is read photometrically. Test performed by VRL Eurofins,  6933 S. Revere Parkway, Centennial, CO 80112. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration. See package insert for more information

Within 24 hours from receipt of specimen (Monday - Friday).

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
Serum 30827 86777 Yes

100 µL (min 50 µL)

Qualitative

  • Collect whole blood in an gold, red, or red/gray tiger top tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped at refrigerated temperature, must be received within 2 days.
  • If not shipping in original container, centrifuge and transfer 100 µL (min 50 µL) serum to screw top tube. Specimens shipped at refrigerated temperature must be received within 2 days of collection, or frozen.
Shipping

All specimens must be labeled with patient's name and collection date. A Viracor/VRL Eurofins Pre-Transplant test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112.

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

 

 

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on VRL Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. VRL Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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