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ABO/Rh, Blood Type

Test Code: 30832

A special account is required to order pre-transplant testing. Contact Client Services or your account executive to set up a pre-transplant account to order this assay. Specimens should not be collected until after account has been created.

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Clinical Utility

ABO, RH
The IH-Card ABO/D(DVI-)+Rev A1,B is intended for the performance of forward and reverse ABO grouping and D (RH1) antigen typing of human red blood samples using the IH-System.

ABO, Anti-A, B, AB
For the determination of the A (ABO1), B (ABO2), A,B (ABO3) antigens of red blood cells using the tube test.

ABO, A1, B, A2
Biotestcell® A1 & B and Biotestcell® A2 are used for the detection of antibodies to A and B antigens in test serum or plasma.

ABO, Modified LISS
MLB 2 is a modified low ionic strength solution used to enhance the detection of red blood cell antibodies and antigens in indirect and direct antiglobulin tests in both tube and solid phase methods with Solidscreen® II on TANGO instruments.

 

Procedure

ABO, RH
The test combines the principles of hemagglutination and gel filtration for detection of blood group antigen-antibody reactions. The test sample (red blood cell suspension and/or plasma/serum) is distributed into the microtubes containing the appropriate reagent(s). After centrifugation non-agglutinated red blood cells are collected at the bottom of the microtube while the agglutinates are dispersed throughout the length of the gel, depending upon their size. Their position in the gel determines the intensity of the reaction. Package Insert 

ABO, Anti-A, B, AB
The test principle is hemagglutination. The antibodies in Seraclone® Anti-A (ABO1), Seraclone® Anti-B (ABO2) and Seraclone® Anti-A,B (ABO3) bind to the corresponding antigen on red blood cells being tested and cause an antigen-antibody reaction visible as red blood cell agglutination. The four ABO blood groups A, B, AB and O are defined by the presence or absence of A and B antigens on red blood cells. The absence of both A and B antigens defines blood type O. The antigens A and B react with the corresponding antibody in Seraclone® Anti-A (ABO1), Seraclone® Anti-B (ABO2), and Seraclone® Anti-A,B (ABO3). Package Insert 

ABO, A1, B, A2
The test principle is a hemagglutination test. The antigens of the Reagent Red Blood Cells react with the respective antibodies in the serum or plasma to be tested.
The existence or lack of Anti-A and/or Anti-B antibodies must correspond with the existence or lack of A and/or B antigens on the Reagent Red Blood Cells. Package Insert 

ABO, Modified LISS
Red blood cells are suspended in MLB 2/Alsevers Solution for crossmatch, DAT, weak D typing and in MLB 2 for antibody detection, -identification to be tested in the solid phase antiglobulin test Solidscreen® II. Package Insert 

Within 24 hours from receipt of specimen.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
Plasma 30832 86900/ 86901 No

2 mL (min 1 mL) 

Qualitative

  • Collect whole blood in an EDTA, lavender top tube. Do not freeze
  • Ship ambient or refrigerated temperature, must be received within 5 days of collection.
  • No ACT or neutral gel separator tubes.
Shipping

All specimens must be labeled with patient's name and collection date. A Viracor/VRL Eurofins Pre-Transplant test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112.

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on VRL Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. VRL Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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