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RBC Antibody (Ab) Screen

Test Code: 30833

A special account is required to order pre-transplant testing. Contact Client Services or your account executive to set up a pre-transplant account to order this assay. Specimens should not be collected until after account has been created.

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Clinical Utility

Capture-R® Ready-Screen® (Pooled Cells) is intended for use in the detection of unexpected IgG antibodies to red blood cells by manual, semiautomated or automated solid phase red blood cell adherence methods. 

About RBC Antibody Screen

Unexpected antibodies are found in the sera of 0.3 to 3% of donor and patient populations.1-3 Many antibodies are of clinical importance since they may cause decreased red blood cell survival as the result of hemolytic transfusion reactions, hemolytic disease of the newborn or autoimmune hemolytic anemia. In vitro antibody detection (screening) tests are employed to reveal the presence of these antibodies in patient and donor sera. Selected red blood cells, such as those provided as Capture-R Ready-Screen, are incubated with test sera or plasma under conditions that will facilitate antibody detection.4 Capture-R Ready-Screen (Pooled Cells) is not recommended for pretransfusion tests done in lieu of a major crossmatch to detect unexpected antibodies in patients' samples. 

Procedure

Capture-R® Ready-Screen is a modified solid phase antibody detection systems based on the procedures of Plapp et al 5 and Juji et al.6 Membranes of red blood cells have been bound to and dried on the surfaces of polystyrene microwells. The membrane antigens are used to capture red blood cell-specific antibodies from patient or donor sera or plasmas. Following a brief incubation period, unbound residual immunoglobulins are rinsed from the wells and replaced with a suspension of anti-IgG-coated indicator red blood cells. Centrifugation brings the indicator red cells in contact with antibodies bound to the reagent red blood cell membranes. In the case of a positive test, the migration of the indicator red blood cells to the bottom of the wells is impeded as anti-IgG-IgG complexes are formed on the surface of the immobilized reagent layer. As a consequence of antibody bridging, the indicator red cells adhere to the screening cells as a second immobilized layer. In the absence of detectable antigen-antibody interactions (negative test), the indicator red blood cells will not be impeded during their migration and will pellet to the bottom of the wells ans tightly agglutinated red cell buttons. See package insert for more information.

Within 24 hours from receipt of specimen (Monday - Saturday).

 

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
plasma 30833 86850 Yes

1 mL (min 0.5 mL)

Qualitative

  • Collect whole blood in an ACD or EDTA tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped at refrigerated temperature.
  • If not shipping in original container, centrifuge and transfer 1 mL (min 0.5 mL) plasma to screw top tube. Ship specimen at refrigerated or frozen temperature.
serum 30833 86850 Yes

1 mL (min 0.5 mL) 

Qualitative

  • Collect whole blood in an red top tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped at refrigerated temperature.
  • If not shipping in original container, centrifuge and transfer 1 ml (min 0.5 mL) serum to screw top tube. Ship specimen at refrigerated or frozen temperature.
Shipping

All specimens must be labeled with patient's name and collection date. A Viracor/VRL Eurofins Pre-Transplant test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112.

 

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on VRL Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. VRL Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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