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RBC Antibody (Ab) Screen

Test Code: 30833

A special account is required to order pre-transplant testing. Contact Client Services or your account executive to set up a pre-transplant account to order this assay. Specimens should not be collected until after account has been created.

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Clinical Utility

RBC Antibody Screen
IH-Cell I-II and IH-Cell I-II-III are intended for the detection of antibodies to human red blood cell antigens in patients and donors. IH-Cell Pool is intended for the detection of antibodies to human red blood cell antigens in donors.

RBC Antibody Screen, Solidscreen® II
Solidscreen® II Negative Control is intended for use as a negative control for the Solidscreen® II assay with TANGO® instruments.

RBC Antibody Screen, Tube Pool Cells
Biotestcell® Pool, Biotestcell® 1 & 2 and Biotestcell® 3 are used for the detection of unexpected antibodies in tube test and solid phase test Solidscreen® II manually.

 

Procedure

Refer to the instructions for use for the specific IH-Card tested with the Reagent Red Blood Cells.

RBC Antibody Screen, Solidscreen® II
Solidscreen® II is a solid phase assay for the detection of red cells sensitized with antibodies in human plasma or serum.
The Solidscreen® II well is coated with Protein A. Protein A is a component of the cell wall of Staphylococcus aureus and has a very high affinity for the Fc portion of most immunoglobulin classes.1
The plasma or serum and Reagent Red Blood Cells are added to the Protein A coated well.
Sensitization of the Reagent Red Blood Cells occurs if the corresponding antibody is present for the antigen on the red cell. Following incubation, and two wash processes to remove unbound protein, Anti-Human Globulin, Anti-IgG Solidscreen® II is added to the well. Following centrifugation, the well is evaluated. A smooth monolayer of cells is indicative of a positive reaction. A compact button of cells in the middle of the well is indicative of a negative reaction. [hyperlink to package insert IFU-187735 Solidscreen II Negative Control]

RBC Antibody Screen, Tube Pool Cells
The test principle is a hemagglutination test or solid phase test. Antigens on the Reagent Red Blood Cells react with the corresponding antibodies in the serum or plasma directly or after addition of Anti-Human Globulin. In a tube test agglutination will occur. In solid phase test Solidscreen® II a uniform layer of red blood cells on the micro test plate wells will occur. [hyperlink to package insert IFU-186184 Tube Pool Cells]

Within 24 hours from receipt of specimen (Monday - Saturday).

 

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
plasma 30833 86850 No

1 mL (min 0.5 mL)

Qualitative

  • Collect whole blood in an ACD or EDTA or Sodium Citrate tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped at ambient or refrigerated temperature.
  • If not shipping in original container, centrifuge and transfer 1 mL (min 0.5 mL) plasma to screw top tube. Ship specimen at refrigerated or frozen temperature.
serum 30833 86850 No

1 mL (min 0.5 mL) 

Qualitative

  • Collect whole blood in an red top tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped at ambient or refrigerated temperature.
  • If not shipping in original container, centrifuge and transfer 1 ml (min 0.5 mL) serum to screw top tube. Ship specimen at refrigerated or frozen temperature.
Shipping

All specimens must be labeled with patient's name and collection date. A Viracor/VRL Eurofins Pre-Transplant test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112.

 

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on VRL Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. VRL Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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