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Syphilis Screening Titer - Nontreponemal (RPR)

Test Code: 30834

A special account is required to order pre-transplant testing. Contact Client Services or your account executive to set up a pre-transplant account to order this assay. Specimens should not be collected until after account has been created.

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Clinical Utility

The ASI RPR (rapid plasma reagin) Card Test for syphilis is a qualitative and semiquantitative nontreponemal flocculation test for the detection of reagin antibodies in human serum and plasma as a screening test for serological evidence of syphilis. This test is also intended for use in screening donor specimens for blood or tissue donation when the test is read and interpreted with the ASiManager-AT. The ASI RPR Card Test for Syphilis is for professional use only. 

About Syphilis Screening

Treponema pallidum, the etiological agent of syphilis, induces the production of at least two types of antibodies in human infection: anti-treponemal antibodies that can be detected by FTA-ABS antigen1, and anti-nontreponemal antibodies (reagin) that can be detected by RPR antigen.2 

Procedure

The ASI RPR Card Test is an 8-minute macroscopic nontreponemal flocculation test to be used for the detection of reagin. The micro - particulate carbon RPR antigen enhances the visual discrimination between reactive and nonreactive results. The reagin-type antibody binds with the antigen that is composed of a complex of cardiolipin, lecithin and cholesterol particles with activated charcoal. The result of this antigen-antibody reaction is macroscopic flocculation. Test performed by VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112. This test has been cleared or approved for diagnostic use by the U.S. Food and Drug Administration. See Package Insert for more information.

Within 24 hours from receipt of specimen (Monday - Friday).

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
plasma 30834 86886 Yes

300 µL (min. 200 µL)

Semi-Quantitative

  • Collect whole blood in an EDA tube. Do not freeze whole blood or plasma.
  • Sample sent in original vacutainer tube can be shipped at refrigerated temperature, must be received within 5 days.
Serum 30834 86886 Yes

300 µL (min. 200 µL)

Semi-Quantitative

  • Collect whole blood in an gold, red, or red/gray tiger top tube. Do not freeze whole blood.
  • Sample sent in original vacutainer tube can be shipped at refrigerated temperature, must be recieved within 5 days.
  • If not shipping in original container, centrifuge and transfer 300 µL (min. 200 µL) serum to screw top tube. Specimens shipped at refrigerated temperature must be received within 5 days of collection, or frozen.

 

 

Shipping

All specimens must be labeled with patient's name and collection date. A Viracor/VRL Eurofins Pre-Transplant test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: VRL Eurofins, 6933 S. Revere Parkway, Centennial, CO 80112.

Causes for Rejection

Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on VRL Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. VRL Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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