Skip to the content

Hepatitis D Virus (HDV) Qualitative Real-time RT-PCR

Test Code: 3900
Expand All Collapse All
Clinical Utility

Hepatitis D virus (HDV) is an incomplete RNA virus that requires the helper function of Hepatitis B virus (HBV) envelope proteins (HBSAg) to replicate.  The HDV Real-time RT-PCR assay detects HDV RNA in serum or plasma. 

Procedure

Extraction of nucleic acid from specimen, followed by reverse transcription of viral RNA, then amplification and detection of cDNA using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

Specificity

The assay is specific for Hepatitis D.

Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
Plasma 3901 87799 Yes

2 mL (min. 0.5 mL)

Detected/Not Detected

  • Collect 4-5 mL whole blood in EDTA or ACD tube.
  • Centrifuge (optional) and transfer 2 mL plasma to sterile, screw top tube.
  • Alternatively plasma can be shipped in the original collection tube without centrifugation.
  • Can be shipped at ambient or refrigerated temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 7 days of collection.

 

 

Serum 3910 87799 Yes

2 mL (min. 0.5 mL)

Detected/Not Detected

  • Collect 4-5 mL whole blood in red top tube.
  • Centrifuge and transfer 2 mL serum to sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient temperature must be received within 7 days of collection.
Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086.

Causes for Rejection

Specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above. The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

References

Price, J. An update on Hepatitis B, D and E Viruses. (2014) Topics in Antiviral Medicine 21(5):157-163.

Connect with us