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T Cell Fx™-Select

Test Code: 403065P

Cell Function Testing requires special attention to specimen collection and shipping in order to ensure the integrity of the sample.  Please watch Viracor’s video for complete specimen collection and shipping instructions.


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Clinical Utility

The test measures the T cell responses to antigens of common infections as well as to mitogens, allowing detection of deficiencies in this arm of the immune response. Patients with primary immunodeficiency may have deficits in the acquired T-cell response. It is useful for the following types of patients: Immune deficiency, Recurrent infection, Evaluation of cell-mediated immunity and monitoring immunosuppression (e.g. Transplant patients).


Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37ºC. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, and CD69 and intracellular IFN-gamma, and analyzed by flow cytometry. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

2-3 business days from receipt of specimen

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
whole blood 403065P 86353 x 2 No

8 mL

Varies by antigen/mitogen

  • 8 mL of whole blood. Collect and ship in original sodium heparin vacutainer.
  • DO NOT USE lithium heparin, ACD tubes or EDTA anticoagulants.
  • Due to time constraints of the T Cell FX™ assay, samples need to be collected between the hours of 12 p.m. and 5 p.m. Central time, Monday thru Thursday.
  • Sample must be received within 24 hours of draw.
  • Ship samples via priority overnight courier at ambient temperature.
  • DO NOT SHIP on days where a holiday follows the shipping or set up day.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for general informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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