HHV-6 Quantitative Real-time PCR
Some specimen types for this assay are reported as qualitative results; please see our Specimen Information section below for more information.
Quantitative HHV-6 DNA PCR can be used for early detection of a primary infection, for tracking the course of infection, and for monitoring response to treatment. Quantitative HHV-6 DNA PCR does not differentiate HHV-6 viremia from patients with chromosomally integrated HHV-6, a relatively uncommon congenital occurrence that has not been conclusively related to a disease state. Treatment of HHV-6 in immunocompromised patients presents challenges, including drug toxicity, delayed onset of disease after discontinuing therapy, and emergence of mutations that may affect the ability of diagnostic assays to detect them efficiently.
HHV-6 reactivation can cause fever, rash, hepatitis, encephalitis, pneumonitis, delay or suppression of bone marrow engraftment and/or increased risk of cytomegalovirus (CMV) infection in bone marrow or solid organ transplant patients. Bone marrow suppression due to HHV-6 infection is often confused with rejection in hematopoietic stem cell transplantation (HSCT) patients.
Extraction of HHV-6 viral DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
Detects both Type A and Type B in one assay. The primers and probes used in this assay are specific for known strains of HHV-6 based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, EBV, HSV-1, HSV-2, HHV-7, HHV-8, JCV, parvovirus B19, SV-40, and VZV.
Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.
|Specimen Type||Order Code||CPT Code||NY Approved||Volume||Assay Range||Special Instructions|
|BAL||6509||87533||Yes||2 mL (min. 0.5 mL)||78 copies/mL to 1x108 copies/mL||
|bone marrow||6504||87533||Yes||2 mL (min. 0.5 mL)||183 copies/mL to 1x108 copies/mL||
|bronch wash||6526||87533||Yes||2 mL (min. 0.5 mL)||78 copies/mL to 1x108 copies/mL||
|CSF||6503||87533||Yes||2 mL (min. 0.5 mL)||81 copies/mL to 1x108 copies/mL||
|fecal||6508||87532||Yes||Size of pea, or 2 mL liquid stool||Detected/Not Detected||
|plasma||6501||87533||Yes||2 mL (min. 0.5 mL)||188 copies/mL to 1x108 copies/mL||
|serum||6510||87533||Yes||2 mL (min. 0.5 mL)||188 copies/mL to 1x108 copies/mL||
|tissue [Qual]||6506||87798||Yes||5 mg fresh tissue (approximately ½ of a pencil eraser size)||Detected/Not Detected||
|tissue [Quant]||6505||87533||Yes||5 mg fresh tissue (approximately ½ of a pencil eraser size)||8 copies/mL to 1x108 copies/mL||
|trach asp||6519||87533||Yes||2 mL (min. 0.5 mL)||78 copies/mL to 1x108 copies/mL||
|trach wash||6548||87533||Yes||2 mL (min. 0.5 mL)||78 copies/mL to 1x108 copies/mL||
|whole blood||6516||87533||Yes||2 mL (min. 0.5 mL)||79 copies/mL to 1x108 copies/mL||
Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086
Specimens beyond their acceptable length of time from collection as listed in the specimen handling, specimens received in trap containers, or specimen types other than those listed.
Specimens are approved for testing in New York only when indicated in the Specimen Information field above.
The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.
PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.