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Influenza A and B qRT-PCR

Test Code: 7700

Influenza A and Influenza B qRT-PCR is available with a self-pay price of $95.80 for patients who do not have health insurance or for those who intentionally choose not to submit a claim to their insurance company. Additional charges may apply for specimen collection, logistics or expedited turnaround testing.

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Clinical Utility

The Influenza A and Influenza B qRT-PCR test is intended for the quantitative detection of Influenza A and Influenza B.

About Influenza A and B

Human Influenza A viruses are medically important pathogens that cause significant morbidity and mortality throughout the world. The emergence of the 2009 H1N1 influenza A virus has led to increased vigilance in monitoring these infections. Unlike Influenza A viruses, Influenza B virus is found only in humans. Influenza B virus is a negative-sense, single-stranded RNA. Type B flu may cause a less severe reaction than type A flu virus, but type B can still be extremely harmful for the immunocompromised.

Procedure

Extraction of Influenza A and B virus nucleic acid from specimen, followed by combined reverse transcription of viral RNA and PCR amplification using real-time reverse transcriptase PCR (RT-PCR) methods. An internal control is added to ensure that extraction was performed correctly and that the RT-PCR reaction was not inhibited.

This test was developed and its performance characteristics determined by Viracor Eurofins. It has not been cleared or approved by the U.S. Food and Drug Administration. Results should be used in conjunction with clinical findings, and should not form the sole basis for a diagnosis or treatment decision.

Specificity

The primers and probes used in the INFA assay are specific for known H3N2, pandemic (2009) H1N1 and seasonal H1N1 and the primers and probes used in the INFB assay are specific for known INFB strains.  Additionally, no cross reactivity was detected when tested against ADV, CMV, EBV, HSV-1, HSV-2, Enterovirus, Parainfluenza virus, Coronavirus 229E, SARS CoV-2, Rhinovirus, RSVA, RSVB,  C. pneumoniae and Group A Strep.  In silico analysis has demonstrated that Viracor’s INFA or INFB assays are not expected to cross-react with other respiratory viruses, bacteria or fungal pathogens/commensals.

During this unprecedented time of high volume, the turnaround time for most samples is 24-48 hours from receipt of specimen in the lab.  As daily volume can have an impact on workflow, we are monitoring this closely and will provide updates as the situation changes.

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
BAL [Quant] 7779 87502 No

2 mL

Flu A: 152 - 1.00E+09 copies/mL

Flu B: 91 - 1.00E+09 copies/mL

  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
BAL [Qual] 7781 87502 No

2 mL

 Detected / Not Detected

  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
bronch wash [Quant] 7779 87502 No

2 mL

Flu A: 152 - 1.00E+09 copies/mL

Flu B: 91 - 1.00E+09 copies/mL

  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
bronch wash [Qual] 7781 87502 No

2 mL

 Detected / Not Detected

  • Collect in a sterile, screw top tube.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
nasal swab [Quant] 7778 87502 No

2.0 mL (1.0 mL)

Flu A: 559 - 1.00E+09 copies/mL

Flu B: 327 - 1.00E+09 copies/mL

  • Place sterile swab in 2 mL sterile buffered saline or transport media in a sterile, screw top tube.
  • Do not use calcium alginate swab or wood shafted swab.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
nasal swab [Qual] 7780 87502 No

2.0 mL (1.0 mL)

Detected / Not Detected

  • Place sterile swab in 2 mL sterile buffered saline or transport media in a sterile, screw top tube.
  • Do not use calcium alginate swab or wood shafted swab.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
NP swab [Quant] 7778 87502 No

2.0 mL (1.0 mL)

Flu A: 559 - 1.00E+09 copies/mL

Flu B: 327 - 1.00E+09 copies/mL

  • Place sterile swab in 2 mL sterile buffered saline or transport media in a sterile, screw top tube.
  • Do not use calcium alginate swab or wood shafted swab.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
NP swab [Qual] 7780 87502 No

2.0 mL (1.0 mL)

Detected / Not Detected

  • Place sterile swab in 2 mL sterile buffered saline or transport media in a sterile, screw top tube.
  • Do not use calcium alginate swab or wood shafted swab.
  • Can be shipped at ambient or frozen temperature Monday through Friday.
  • Specimens shipped at ambient or refrigerated temperature must be received within 7 days of collection, or 28 days when frozen.
Shipping

Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. For additional testing, please send a separate specimen. Refer to our website for accepted specimen types. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086.

Causes for Rejection

Dry swab, wood shafted swab, calcium alginate swab, specimens received in trap containers, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

We will continue to follow federal and state requirements for both notification of results and any confirmatory testing that is required by another agency.

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